AFLP GENETIC MAPS OF EUCALYPTUS-GLOBULUS AND EUCALYPTUS-TERETICORNIS

Amplified fragment length polymorphism (AFLP) analysis is a rapid and efficient technique for detecting large numbers of DNA markers in euca lypts. We have used AFLP markers in a two-way pseudo-testcross strateg y to generate genetic maps of two clones of different Eucalyptus speci es (E. tereticornis and E. globulus). Of 606 polymorphic fragments sco red, 487 segregated in a 1:1 ratio, corresponding to DNA polymorphisms heterozygous in one parent and null in the other. In the maternal E. tereticornis map, 268 markers were ordered in 14 linkage groups (919 c M); the paternal E. globulus map had 200 markers in 16 linkage groups (967 cM). Results from PGRI software were compared with MAPMAKER. The average density of markers was approximately 1 per 3.9 cM. Framework m arkers were ordered with an average confidence level of 90%, covering 80-100% of the estimated Eucalyptus genome size. In order to investiga te the homologies between the E. tereticornis and the E. globulus gene tic linkage maps, we included 19 markers segregating 3:1 in the analys is. Some homeologous linkage groups were recognized. The linkage data developed in these maps will be used to detect loci controlling commer cially important traits.