Tbx-2, Tbx-3, Tbx-4 and Tbx-5 chick genes have been isolated and, like
the mouse homologues, are expressed in the limb regions. Tbx-2 and Tb
x-3 are expressed in anterior and posterior domains in wings and legs,
as well as throughout the flank. Of particular interest, however, are
Tbx-5, which is expressed in wing and flank but not leg, and Tbx-4, w
hich is expressed very strongly in leg but not wing. Grafts of leg tis
sue to wing and wing tissue to leg give rise to toe-like or wing-like
digits in wing and leg respectively. Expression of Tbx-4 is stable whe
n leg tissue is grafted to wing, and Tbx-5 expression is stable when w
ing tissue is grafted to leg. Induction of either extra wings or legs
from the flank by applying FGF-2 in different positions alters the exp
ression of Tbx-4 and Tbx-5 in such a way that suggests that the amount
of Tbx-4 that is expressed in the limb determines the type that will
form. The ectopic limb always displays a limb-like Tbx-3 expression. T
hus Tbx-4 and Tbx-5 are strong candidates for encoding 'wingness' and
'legness'.