The potential environmental hazard of sediment samples from Copenhagen
Harbour was investigated by a combination of chemical analyses and bi
ological tests. The chemical analyses comprised determination of the c
ontent of heavy metals, polycyclic aromatic hydrocarbons and polychlor
inated biphenyls, and the biological tests comprised whole sediment bi
oassays with the amphipod Corophium volutator, testing of a sediment s
uspension with the Microtox Solid Phase test, and testing of sediment
pore water by the copepod Acartia tonsa and the alga Skeletonema costa
tum. For all sediment samples, the concentrations of contaminants exce
eded the Probable Effect Levels and toxic effects should therefore be
expected. However, various degrees of toxicity were determined by the
biotests with the Microtox Solid Phase test system being the most sens
itive, the amphipod and the copepod being intermediately sensitive, an
d no toxicity of the pore water was registered to the algae. Moreover,
no direct correlation between the content of the contaminants in the
sediment samples and the registered toxicity could be established. It
is therefore concluded that the combination of biotesting and chemical
analysis is a valuable tool for evaluating the environmental risks of
sediments. (C) 1998 IAWQ. Published by Elsevier Science Ltd.