IN-SITU DETECTION OF INSULIN-LIKE-GROWTH-FACTOR-II (IGF2) AND H19 GENE-EXPRESSION IN HEPATOCELLULAR-CARCINOMA

To assess the relationship between insulin-like growth factor II (IGF2 ) and H19 gene expression at the cellular level, we have examined the distribution of IGF2 and H19 mRNA by means of in situ hybridization in hepatic malignancies consisting of hepatocellular carcinoma (HCC), ch olangiocellular carcinoma (CCC), and metastatic liver cancer (MLC). In HCC, 15 of 27 tumors (56%) and 11 of 27 tumors (41%) demonstrated inc reased IGF2 and H19 gene expression, respectively. Of 16 HCCs with inc reased expression of either IGF2 or H19, 10 tumors coexpressed both tr anscripts at comparable levels. Moreover, the spatiotemporal distribut ion and the cellular localization of the two gene transcripts were alm ost identical, suggesting the presence of a reciprocal relation betwee n IGF2 and H19. In addition, 5 HCCs showed increased IGF2 expression w ithout concomitant H19 expression, whereas 1 HCC showed increased H19 expression without IGF2 transcripts. However, 11 HCCs showed no IGF2 o r H19 expression. On the other hand, neither IGF2 transcripts nor H19 transcripts were detected in 2 CCCs or 10 MLCs studied. The data sugge st that IGF2 and/or H19 gene expression may be characteristic of some HCCs.