GRADIENT ELUTION IN PREPARATIVE REVERSED-PHASE LIQUID-CHROMATOGRAPHY

Reversed-phase gradient elution is becoming a method of choice for the laboratory-scale preparative separation of complex mixtures and for t he industrial-scale purification of biopolymers. Understanding of band broadening under overloaded conditions in gradient elution is also de veloping and many papers based on the simulation of elution profiles h ave recently been published. They are reviewed with emphasis on the re sulting optimization schemes for gradient elution preparative separati ons. The specific problems connected with the preparative chromatograp hy of peptides and proteins under gradient elution conditions are not discussed.