OPTIMIZATION OF INDUSTRIAL-SCALE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY APPLICATIONS USING A NEWLY DEVELOPED SOFTWARE

Industrial-scale HPLC applications have been evaluated and optimized t o give the desired purity and throughput at the lowest total cost in t he HPLC step by the use of a newly developed software, including the a lgorithm Nirvana. The algorithm, capable of handling self-displacement effects, has been utilized to simulate band profiles and yield in the optimization process. It is shown that this method can be used succes sfully for finding the optimal chromatographic conditions also for app lications with self-displacement.