Sh. Nelson et al., PREGNANCY AUGMENTS NITRIC OXIDE-DEPENDENT DILATOR RESPONSE TO ACETYLCHOLINE IN THE HUMAN UTERINE ARTERY, Human reproduction, 13(5), 1998, pp. 1361-1367
The influence of pregnancy on the dilator effects of acetylcholine in
the isolated human uterine artery was investigated. Acetylcholine (0.1
nM to 0.1 mu M) produced concentration-and endothelium-dependent rela
xation of norepinephrine (3 mu M)-induced contraction, The relaxation
was greater in arteries from pregnant patients (P arteries) than from
non-pregnant patients (NP arteries). The maximal relaxation was 53.5 /- 3.4% (n = 21) in P arteries and 23.5 +/- 2.5% (n = 35) in NP arteri
es. In both P and NP arteries the cholinergic relaxation was increased
in the presence of superoxide dismutase and greatly reduced in the pr
esence of the nitric oxide synthase inhibitors, N-G-mono-methyl L-argi
nine (L-NMMA) and L-nitro-arginine-methylester (L-NAME). The effect of
these nitric oxide synthase inhibitors was reversed by L-arginine. We
conclude that pregnancy enhances acetylcholine-induced nitric oxide s
ynthesis and release in the human uterine artery.