GENOMIC STRUCTURE OF UNP, A MURINE GENE ENCODING A UBIQUITIN-SPECIFICPROTEASE

The murine Unp gene encodes a ubiquitin-specific protease, a member of a family of enzymes that includes the product of the human tre-2 onco gene. The Unp gene has previously been mapped to chromosome 9. We have cloned in bacteriophage a 50 kilobase region of chromosome 9 containi ng the Unp gene, and have determined the nucleotide sequence of the ge ne. The gene has 22 exons, distributed over 47.4 kb. A processed ribos omal S2 pseudogene was identified in the third intron of the Unp gene. Expression of Unp is driven by a GC-rich, 'housekeeping' type promote r. (C) 1998 Elsevier Science B.V. All rights reserved.