EFFECT OF INCORPORATION OF HYDROPHOBIC, HYDROPHILIC AND IONIC GROUPS THROUGH LYSINE MODIFICATION OF BETA-LACTOGLOBULIN ON ITS ADSORPTION ATALUMINA WATER INTERFACE/

Lysine residues offer a convenient way of incorporating various chemic al groups on to the surface of proteins. The effect of introducing che mical groups of various nature on the protein surface on its adsorptio n properties at the alumina/water interface have been studied. Hydroph ilic sugar groups have been introduced by glycosylation, hydrophobic m ethyl group has been introduced by reductive alkylation and negatively charged phosphate group has been introduced by pyridoxylation of beta -lactoglobulin. The adsorption density of these modified proteins on a lumina were compared with that of the unmodified protein. It has been found that all of the above modifications significantly reduced the af finity of beta-lactoglobulin for the solid surface at all the three pH 's studied here.