VARIATION IN THE PROREGION STRUCTURE OF HEPARIN-BINDING EGF-LIKE GROWTH-FACTOR PRECURSORS

In a previous study, we have isolated and characterized cDNA encoding a novel 'short form' of heparin-binding EGF-like growth factor (SF HB- EGF) (Loukianov et al., 1997). In the present work, we have found that cDNA for SF HB-EGF and for full-length HB-EGF are each represented by two variants, which we refer to as L and P forms. The L form is the p reviously known form of HB-EGF cDNA and encodes a leucine in position 33. The P form described in this report, encodes a proline in codon 33 . The L33P substitution is predicted to cause a significant alteration in the proregion structure of SF HB-EGF and HB-EGF. (C) 1998 Elsevier Science B.V. All rights reserved.