Y. Takamatsu et al., IDENTIFICATION OF AN ALTERNATIVE FORM OF THE DROSOPHILA CA2-DEPENDENTPROTEIN-KINASE-II THAT IS MATERNALLY DERIVED( CALMODULIN), Biochimica et biophysica acta. Molecular cell research, 1220(2), 1994, pp. 188-192
Four forms of the Drosophila Ca2+/calmodulin-dependent protein kinase
II are generated from a single gene by alternative splicing (Ohsako et
al. (1993) J. Biol. Chem. 268, 2052-2062). We identified a fifth form
of the cDNA encoding the enzyme expressed in the ovary, unfertilized
egg and early embryos by reverse transcription-polymerase chain reacti
on, which suggests that it is maternally derived. The fifth form was a
lso generated from the gene by alternative splicing and was identical
to the cDNA encoding the 530-amino-acid polypeptide, the longest of th
e four forms previously identified, except that it lacked exon 11. Thr
ee splicing derivatives which lost one amino acid from the 509- and 53
0-amino-acid polypeptides were also found in 4 to 10 h embryos.