ALTERNATIVE PROMOTER USAGE AND TISSUE-SPECIFIC EXPRESSION OF THE MOUSE SOMATOSTATIN RECEPTOR-2 GENE

We have cloned the 5' upstream regulatory region of the mouse somatost atin receptor 2 gene. Its genomic organization is novel among all soma tostatin receptor genes. It contains two previously unrecognized exons , separated by introns larger than 25 kb, and three tissue and cell sp ecific alternative promoters, The first promoter in front of exon 1 is active only in AtT-20 tumor cells. The second promoter, located 5' to exon 2, is used in brain, pituitary, adrenals, pancreas, NG 108-15 an d AtT-20 cells. Furthermore, it contains putative DNA elements for reg ulation by glucocorticoids, estradiol and cAMP. A third promoter, loca ted in exon 3, is additionally used in lung, kidney and spleen. (C) 19 98 Federation of European Biochemical Societies.