EFFECTS OF TRIMETAZIDINE ON LIPID-PEROXIDATION AND PHOSPHORUS METABOLITES DURING GOLD STORAGE AND REPERFUSION OF ISOLATED-PERFUSED RAT KIDNEYS

This study was undertaken to evaluate the effect of trimetazidine (TMZ ) during cold storage (CS) and the consequence during normothermic rep erfusion in an isolated perfused rat kidney model (IPK), IPK was used to assess the rate of perfusion flow, the ratio of ATP to inorganic ph osphate (ATP/Pi) as a reflection of the energetic status during reperf usion, intracellular pH (pH(i)), tissue water content and malondialdeh yde (MDA) tissue levels in four different preservation solutions after 48-hr preservation at 4 degrees C and 2-hr reperfusion at 37.5 degree s C: EuroCollins (EC), University of Wisconsin (UW), EC plus TMZ (10(- 6) M) (EC + TMZ) and UW plus TMZ (10(-6) M) (UW + TMZ). When TMZ was a dded to the preservation solutions, perfusion flow rate (PFR) was sign ificantly improved during reperfusion. Tissue water content, which ref lected tissue edema, was significantly lower in TMZ groups than in gro ups without TMZ during both CS and reperfusion conditions. In TMZ grou ps, ATP/Pi ratio was also significantly improved during CS and reperfu sion. In addition, TMZ lowered the pH(i) both during preservation and after reperfusion. MDA renal tissue level significantly decreased with TMZ both during the preservation period and after reperfusion, These overall results strongly suggested that TMZ contributes to renal prote ction from cold ischemia-reperfusion injury in this IPK model, especia lly when TMZ was added to UW solution and during prolonged hypothermic ischemia.