LONG-ACTING BLOCKADE OF BIOGENIC-AMINE TRANSPORTERS IN RAT-BRAIN BY ADMINISTRATION OF THE POTENT NOVEL TROPANE 2-BETA-PROPANOYL-3-BETA-(2-NAPHTHYL)-TROPANE
Jb. Daunais et al., LONG-ACTING BLOCKADE OF BIOGENIC-AMINE TRANSPORTERS IN RAT-BRAIN BY ADMINISTRATION OF THE POTENT NOVEL TROPANE 2-BETA-PROPANOYL-3-BETA-(2-NAPHTHYL)-TROPANE, The Journal of pharmacology and experimental therapeutics, 285(3), 1998, pp. 1246-1254
2 beta-Propanoyl-3 beta-(2-naphthyl)-tropane (WF-23) is a potent cocai
ne analog with activity at dopamine and serotonin transporters. The pu
rpose of these experiments was to characterize the lime course of effe
cts of acute administration of WF-23 on spontaneous locomotion and bio
genic amine transporters. Rats received injections i.p, with WF-23 (1
mg/kg), cocaine (30 mg/kg) or vehicle and locomotor activity was measu
red at various times postinjection. Animals were killed immediately af
ter behavioral activity. Locomotor activity was significantly increase
d by WF-23 administration, reaching maximum at 4 hr and persisting for
24 hr. Cocaine-elicited elevations in locomotor activity occurred onl
y at the earliest times. WF-23 decreased DA transporter binding in str
iatal membranes ([I-125]RTI-55 binding), with >50% loss in binding for
up to 49 hr postinjection. WF-23 increased the K-d of the high affini
ty site, with no effect on B-max. Cocaine depressed binding (20%) only
at the earliest times. WF-23 decreased levels of [H-3]WIN 35,428 bind
ing sites up to 95% of control in both dorsal and ventral striatum wit
h a similar time-course when assessed autoradiographically. WF-23 also
reduced [H-3]citalopram binding to serotonin transporter sites throug
hout the brain. The slow onset and very long duration of action of WF-
23, taken together with its actions at dopamine and serotonin transpor
ters, suggest a potential role for treatment of disorders characterize
d by their involvement of these neural systems.