TRANSFUSION OF PHOSPHOENOLPYRUVATE-TREATED BLOOD INCREASES OXYGEN-CONSUMPTION IN ACUTE HEMORRHAGE

BACKGROUND: Incubating blood with phosphaenol-pyruvate decreases hemog lobin oxygen affinity (HOA). This study compared transfusion with phos phoenolpyruvate-treated blood and conventionally stored blood on oxyge n consumption in acutely anemic dogs. METHODS: Dogs underwent isovolem ic hemodilution (hematocrit = 10%). After 1 hour they were transfused to a hematocrit of 18% with control or phosphoenolpyruvate treated blo od. Cardiac output, co-oxymetry, and hemoglobin P-50 measurements allo wed calculation of oxygen consumption during anemia, and posttransfusi on. RESULTS: Hemodilution doubled cardiac output. Transfusion with pho sphoenolpyruvate-treated blood allowed greater O-2 consumption than co ntrol (8.31 +/- 2.1 and 3.73 +/- 0.11 cc/kg/mm). There were no differe nces in arterial or venous pO(2) or pH; there were marked differences in HOA, measured by posttransfusion P-50 (21 +/- 3 versus 47 +/- 4), a nd mixed venous O-2 saturation. CONCLUSIONS: Decreased HOA results in increased O-2 consumption in dogs subjected to anemic hypoxia. Phospho enolpyruvate-treated blood provides increased oxygen consumption at a similar hematocrit when compared with untreated banked blood. (C) 1998 by Excerpta Medica, Inc.