QUANTIFICATION OF HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 RNA LEVELS IN PLASMA BY USING SMALL-VOLUME-FORMAT BRANCHED-DNA ASSAYS

Authors
YEGHIAZARIAN T ZHAO WQ READ SE KABAT W LI XY HAMREN SJ SHERIDAN PJ WILBER JC CHERNOFF DN YOGEV R
Citation
T. Yeghiazarian et al., QUANTIFICATION OF HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 RNA LEVELS IN PLASMA BY USING SMALL-VOLUME-FORMAT BRANCHED-DNA ASSAYS, Journal of clinical microbiology, 36(7), 1998, pp. 2096-2098
Citations number
7
Categorie Soggetti
Microbiology
Journal title
Journal of clinical microbiology → ACNP
ISSN journal
00951137
Volume
36
Issue
7
Year of publication
1998
Pages
2096 - 2098
Database
ISI
SICI code
0095-1137(1998)36:7<2096:QOHTRL>2.0.ZU;2-R
Abstract
We have developed small-volume (50 or 250 mu l)-format branched-DNA as says for human immunodeficiency virus type 1 (HIV-1) RNA for use with specimens in which the volume is limited and/or a high viral load is a nticipated. These formats exhibited good correlation with the standard 1-ml format; high specificity, reproducibility, and linearity; and no significant difference in the quantification of HIV-1 subtypes.