COMPARISON OF SAMPLE PREPARATION METHODS FOR DETECTION OF LEGIONELLA-PNEUMOPHILA IN CULTURE-POSITIVE BRONCHOALVEOLAR LAVAGE FLUIDS BY PCR

Authors
JAULHAC B REYROLLE M SODAHLON YK JARRAUD S KUBINA M MONTIEL H PIEMONT Y ETIENNE J
Citation
B. Jaulhac et al., COMPARISON OF SAMPLE PREPARATION METHODS FOR DETECTION OF LEGIONELLA-PNEUMOPHILA IN CULTURE-POSITIVE BRONCHOALVEOLAR LAVAGE FLUIDS BY PCR, Journal of clinical microbiology, 36(7), 1998, pp. 2120-2122
Citations number
20
Categorie Soggetti
Microbiology
Journal title
Journal of clinical microbiology → ACNP
ISSN journal
00951137
Volume
36
Issue
7
Year of publication
1998
Pages
2120 - 2122
Database
ISI
SICI code
0095-1137(1998)36:7<2120:COSPMF>2.0.ZU;2-H
Abstract
A prospective study was conducted on 25 Legionella pneumophila culture -positive and 98 culture-negative bronchoalveolar lavage fluid samples to compare two DNA preparation methods: a rapid modified Chelex-based protocol and a proteinase K method. PCR was found to be more sensitiv e with the Chelex-based method (P = 0.03). No difference was found con cerning the inhibition rate.