Fd. Rubens et al., THE IMPORTANCE OF SAMPLING SITE IN THE MEASUREMENT OF WHOLE-BLOOD PLATELET FLOW-CYTOMETRY, Journal of cardiothoracic and vascular anesthesia, 12(3), 1998, pp. 309-313
Citations number
17
Categorie Soggetti
Anesthesiology,"Peripheal Vascular Diseas","Cardiac & Cardiovascular System
Purpose: Flow cytometry is an emerging technology that may be of use i
n clarifying the defects of platelet function after cardiopulmonary by
pass. However, the technique used for platelet sampling may affect res
ults. The objective of this study was to evaluate the influence of the
sampling site on the degree of expression of a variety of platelet-as
sociated proteins. Methods: Whole-blood flow cytometric assays for the
detection of platelet glycoprotein (GP) Ib, guanosine monophosphate(G
MP)-140, thrombospondin, activated GPIIb/IIIa. and platelet-associated
factor (FXIIIa) were developed. These markers were then measured in s
amples taken simultaneously from a peripheral vein, radial artery, and
the side port of the central venous catheter, in eight patients about
to undergo surgery. Results: When multiple samples from individual pa
tients were assessed, the degree of activation with all of the activat
ion assays (GMP-140, thrombospondin, activated GPIIb/IIIa, FXIIIa) was
significantly greater in samples taken from the arterial catheter (p
< 0.05) compared with the central venous catheter or the peripheral ve
in. The mean difference between sample sites was calculated in the stu
dy patients. Percent activation of FXIIIa from arterial blood was sign
ificantly greater than from the central vein and the peripheral vein (
arterial-peripheral venous, 18.7 +/- 8.6; central venous-peripheral ve
nous, 3.7 +/- 3.6; p = 0.005). There was no site-related difference in
the detected expression of platelet GPIb. Conclusion: The site of pla
telet sampling significantly affects the degree of activation detected
by flow cytometry. To approximate results that would be obtained from
peripheral blood, samples should be taken from the side port of the c
entral venous catheter and not from the arterial catheter in patients
studied during surgery. Copyright (C) 1998 by W.B. Saunders Company.