Atherosclerosis is a complex physiopathologic process initiated by the
formation of cholesterol-rich lesions in the arterial wall. Macrophag
es play a crucial role in this process because they accumulate large a
mounts of cholesterol esters (CEs) to form the foam cells that initiat
e the formation of the lesion and participate actively in the developm
ent of the lesion: Therefore, prevention or reversal of CE accumulatio
n in macrophage foam cells could result in protection from multiple pa
thological effects. In this report, we show that the CE hydrolysis cat
alyzed by neutral cholesterol ester hydrolase (nCEH) can be modulated
by overexpression of hormone-sensitive lipase (HSL) in macrophage foam
cells. For these studies, RAW 264.7 cells, a murine macrophage cell l
ine, were found to be a suitable model of foam cell formation. HSL exp
ression and nCEH activity in these cells and in peritoneal macrophages
were comparable. In addition, antibody titration showed that essentia
lly all nCEH activity in murine macrophages was accounted for by HSL.
To examine the effect of HSL overexpression on foam cell formation, RA
W 264.7 cells were stably transfected with a rat HSL cDNA. The resulti
ng HSL overexpression increased hydrolysis of cellular CEs 2- to 3-fol
d in lipid-laden cells in the presence of an acyl coenzyme A:cholester
ol acyltransferase (ACAT) inhibitor. Furthermore, addition of cAMP pro
duced a 5-fold higher rate of CE hydrolysis in cholesterol-laden, HSL-
overexpressing cells than in control cells and resulted in nearly comp
lete hydrolysis of cellular CEs in only 9 hours, compared with <50% hy
drolysis in control cells. Thus, HSL overexpression stimulated the net
hydrolysis of CEs, leading to faster hydrolysis of lipid deposits in
model foam cells. These data suggest that HSL overexpression in macrop
hages, alone or in combination with ACAT inhibitors, may constitute a
useful therapeutic approach for impeding CE accumulation in macrophage
s in vivo.