RECONSTITUTION OF THE B800 BACTERIOCHLOROPHYLLS IN THE PERIPHERAL LIGHT-HARVESTING COMPLEX B800-850 OF RHODOBACTER-SPHAEROIDES-2.4.1 WITH BCHL A AND MODIFIED (BACTERIO-)CHLOROPHYLLS
M. Bandilla et al., RECONSTITUTION OF THE B800 BACTERIOCHLOROPHYLLS IN THE PERIPHERAL LIGHT-HARVESTING COMPLEX B800-850 OF RHODOBACTER-SPHAEROIDES-2.4.1 WITH BCHL A AND MODIFIED (BACTERIO-)CHLOROPHYLLS, Biochimica et biophysica acta. Bioenergetics, 1364(3), 1998, pp. 390-402
A method is described for reversibly removing bacteriochlorophyll from
the B800-site of the B850-850 antenna complex from Rhodobacter sphaer
oides. This method uses the oligosaccharidic detergent Triton BG-10, t
ogether with an incubation at pH 5.0, Reconstitution at the B800-site
has been successfully achieved for a range of modified bacteriochlorop
hylls. (C) 1998 Elsevier Science B.V. All rights reserved.