D. Broutyboye et V. Magnien, MYOFIBROBLAST AND CONCURRENT ED-B FIBRONECTIN PHENOTYPE IN HUMAN STROMAL CELLS CULTURED FROM NONMALIGNANT AND MALIGNANT BREAST-TISSUE, European journal of cancer, 30A(1), 1994, pp. 66-73
Primary cultures of stromal cells from non-malignant and malignant bre
ast tissues contained myofibroblasts based on immunoreactivity to alph
a-smooth muscle (alpha-sm) actin. The proportions of these cells were
variable among cultures from non-malignant origin while consistently h
igh in cultures from carcinomas. High expression of ED-B fibronectin a
nd of type V collagen was observed in myofibroblast-containing culture
s. While cells from nonmalignant tissues grew relatively steadily, the
proliferation of carcinoma-derived cells declined during serial subcu
lturing. In both types of cultures, alpha-sm actin and ED-B fibronecti
n expression decreased with increasing passage numbers. Epidermal grow
th factor (EGF), fibroblast growth factor b (FGFb), transforming growt
h factor alpha (TGF alpha) and platelet-derived growth factor (PDGF) s
howed consistent mitogenic effects. Addition of FGFb prolonged culture
growth and allowed alpha-sm actin and ED-B fibronectin expression to
persist. These results demonstrate similar phenotypic modulations in s
tromal cells from non-malignant and malignant breast tissues that may
reflect a common stromal response to various tissue injuries, includin
g neoplasia.