THE PREDOMINANT EIF4G-SPECIFIC CLEAVAGE ACTIVITY IN POLIOVIRUS-INFECTED HELA-CELLS IS DISTINCT FROM 2A PROTEASE

Human enteroviruses and rhinoviruses rapidly and selectively abolish t ranslation from cellular mRNA upon infection of susceptible cells. Exp ression of the poliovirus 2A protease (PV 2A(prc)) is sufficient to ca use host translation shutoff through cleavage of eIF4G (formerly p220, eIF4 gamma) either directly or indirectly through activation of a cel lular factor. Evidence exists for both direct and indirect cleavage me chanisms; however, factors presumed to participate in an indirect mech anism have not yet been purified or defined. Here we show that the dom inant eIF4G cleavage activity in lysates from infected HeLa cells was separable from PV 2A(pro) by size exclusion chromatography. 2A(pro) se parated into two peak fractions which contained activity which cleaved a peptide substrate derived from the poliovirus polyprotein. These pe ak 2A(pro) fractions did not cleave eIF4G or an eIF4G-derived peptide, as expected, due to the poor efficiency of direct cleavage reactions, Conversely, fractions which contained peak eIF4G cleavage activity an d only trace amounts of 2A(pro) efficiently cleaved a peptide substrat e derived from the previously mapped eIF4G cleavage site and also clea ved a peptide derived from the poliovirus 1D2A region. The dominant eI F4G cleavage activity was highly purified through four chromatography steps and found to be devoid of all traces of 2A(pro) or its precursor s. Quantitation of 2A(pro) from lysates of infected cells showed that during infections in HeLa cells, 2A(pro) does not reach molar excess o ver eIF4G, as previously shown to be required for direct eIF4G cleavag e in vitro. Further, infection of HeLa cells in the presence of 2 mM g uanidine-HCl, a potent inhibitor of viral RNA replication, suppressed accumulation of 2A(pro) and its precursor 2ABC below detectable levels but was unable to delay the onset of eIF4G proteolysis in vivo. The e IF4G cleavage activity was still easily detectable in in vitro assays using fractions from guanidine-treated cells. Thus, the data suggest t hat poliovirus utilizes two catalytic activities to ensure rapid cleav age of eIF4G in vivo. Although it was not directly measurable here, 2A (pro) likely does cleave a portion of eIF4G in cells. However, the dat a suggest that a cellular factor which can be activated by small quant ities of 2A(pro) constitutes the bulk of the eIF4G-specific cleavage a ctivity in infected cells and is responsible for the rapid and efficie nt eIF4G cleavage activity observed in vivo. (C) 1998 Academic Press.