TECHNIQUES FOR SELECTIVE ISOLATION, SEMIQUANTIFICATION AND RAPID VIRULENCE TESTING OF CATENARIA-ANGUILLULAE

Techniques for selective isolation, semiquantification and rapid virul ence testing are described for Catenaria anguillulae, an important end oparasitic fungus of nematodes. Selective isolation was achieved by us ing second-stage juveniles of Anguina tritici as bait in soil aliquots for 48 h. Semiquantitative estimation of the population of the fungus was made by calculating the infection percentage of inoculated juveni les. Rapid virulence testing was done by using second-stage juveniles of Heterodera cajani, Meloidogyne incognita and A. tritici.