DETECTION OF MULTIPLE RIBOSOMAL-RNA-ITS REGIONS IN ISOLATES OF ASCOCHYTA

Internally transcribed spacer (ITS) regions of the rRNA gene cluster i n isolates of Ascochyta spp, were amplified and digested with restrict ion endonucleases. Addition of fragment sizes from conventional gel el ectrophoresis gave anomalous sizes which suggested the presence of mor e than one PCR product. Separation in HA-Yellow containing agarose gel allowed the detection of fragments of the same size but with differen t sequences. Isolates of Ascochyta spp. were shown to possess multiple PCR-amplification products of the same sizes, but with significant se quence differences.