T. Marting et Jj. Breen, ENHANCEMENTS IN MICELLAR ELECTROKINETIC CHROMATOGRAPHIC SEPARATIONS WITH DECANONYL-N-METHYLALKANAMIDE MICELLES THROUGH THE ADDITION OF ALKYL SULFATES, Journal of chromatography, 808(1-2), 1998, pp. 237-245
Citations number
28
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
Sodium dodecyl sulfate (SDS) and sodium tetradecyl sulfate (STS) are e
ffective additives for improving the efficiency of the micellar electr
okinetic chromatography (MEKC) with micelle forming solutions of decan
onyl-N-methylglucamide (MEGA 10) with boric acid, pH 10. MEGA 10, a no
nionic surfactant, is one of a series of micelle forming compounds for
which the surface charge density of the micelle and electrophoretic m
igration behavior can be varied through the extent of complexation wit
h a charged species, i.e., berate or boronate ions. Using aniline and
a series of 11 substituted anilines as model solutes, the addition of
0-10 mM concentrations of SDS or 0-8 mM STS to buffers containing 20 m
M or 40 mM MEGA 10 and boric acid (1:4 concentration ratio) results in
an increase in the solute capacity factors and an improvement in sepa
ration efficiencies with only small changes in the rate of electroosmo
tic flow and size of the elution range. Further addition of the sodium
alkyl sulfate surfactants results in a large expansion of the elution
range. The effects of SDS and STS are attributed to changes in the st
ructure of the MEGA 10/borate micelles resulting in a mixed surfactant
micelle structure which is more permeable for the solutes. (C) 1998 E
lsevier Science B.V. All rights reserved.