QUALITATIVE DETERMINATION OF INDOLE ALKALOIDS, TRITERPENOIDS AND STEROID OF TABERNAEMONTANA-HILARIANA

This paper reports the separation and identification of indole alkaloi ds, steroids and triterpenoids from the ethanolic extracts of Tabernae montana hilariana (Apocynaceae). The alkaloidal fractions from the eth anolic extracts obtained (root barks, green fruits, ripe fruits and se eds) were fractionated and analysed by thin-layer chromatography, capi llary gas chromatography-flame ionization detection (cGC-FID) as well as by high-resolution gas chromatography-mass spectrometry (HRGC-MS). 3-Hydroxycoronaridine, ibogamine, coronaridine pseudoindoxyl, coronari dine, catharanthine, voacangine hydroxyindolenine, voacangine pseudoin doxyl, tabernanthine, tetraphyllicine, 3-hydroxyvoacangine, voacangine , isovoacangine and 3-oxocoronaridine were identified. The insoluble f raction of ethanolic extracts obtained from the root barks and green f ruits were analysed and ten aliphatic constituents were also identifie d by cGC-FID and HRGC-MS. (C) 1998 Elsevier Science B.V. All rights re served.