Hw. Liu et al., CHARACTERIZATION OF TRICHOMONAS-VAGINALIS VIRUS PROTEINS IN THE PATHOGENIC PROTOZOAN T-VAGINALIS, Archives of virology, 143(5), 1998, pp. 963-970
The 4.6-kb double-stranded (ds) RNA of Trichomonas vaginalis virus (TV
V)-T1 has been shown to encode two overlapping genes, cap and pol. In
this study, a serum for specifically detecting viral cap gene product
was raised against a recombinant protein, and sera for specifically de
tecting pol gene product were raised against synthetic oligopeptides.
A 75-kDa major protein and a 160-kDa minor protein were detected by an
ti-CAP serum in a TVV-T1 sample, indicating that the 75-kDa protein is
the viral capsid protein. The 160-kDa protein alone was also detected
by two distinct anti-POL sera, indicating that the pol gene is expres
sed as a CAP-POL fusion protein. These results suggest that the TVV-T1
genome is arranged into a cap-pol organization in a manner similar to
that of viruses in family Totiviridae.