Re. Horch et al., SINGLE-CELL SUSPENSIONS OF CULTURED HUMAN KERATINOCYTES IN FIBRIN-GLUE RECONSTITUTE THE EPIDERMIS, Cell transplantation, 7(3), 1998, pp. 309-317
To overcome common disadvantages of standard cultured epidermal sheet
grafts (CEG) we have developed a new technique of transplanting cultur
ed human keratinocytes suspended as single cells in a fibrin-glue matr
ix (Keratinocyte fibrin-glue suspension-KFGS). In an athymic mouse mod
el with reproducible standardized full thickness wounds this new techn
ique was compared directly to CEG, Reepithelialization was similar in
both groups, but reconstitution of the dermo-epidermal junction zone,
as shown by electron microscopy and immunohistochemistry was significa
ntly enhanced by the fibrin-glue suspension technique. The new KFGS te
chnique is earlier available than sheet grafts, is able to transfer ac
tively proliferative single keratinocytes, and simplifies the applicat
ion. (C) 1998 Elsevier Science Inc.