VIRUSES PRODUCED FROM COMPLEMENTARY-DNA OF VIRULENT AND AVIRULENT STRAINS OF SWINE VESICULAR DISEASE VIRUSES RETAIN THE IN-VIVO AND IN-VITRO CHARACTERISTICS OF THE PARENTAL STRAIN
T. Kanno et al., VIRUSES PRODUCED FROM COMPLEMENTARY-DNA OF VIRULENT AND AVIRULENT STRAINS OF SWINE VESICULAR DISEASE VIRUSES RETAIN THE IN-VIVO AND IN-VITRO CHARACTERISTICS OF THE PARENTAL STRAIN, Archives of virology, 143(6), 1998, pp. 1055-1062
A full-length cDNA copy of the genome of the pathogenic strain, J1'73,
of swine vesicular disease virus (SVDV) was constructed and inserted
into the plasmid pSVL to generate a recombinant plasmid pSVLSJ1. Infec
tious virus was produced following transfection of cultured mammalian
cells with the plasmid. The recovered virus had the same in vitro prop
el-ties as the parental strain with regard to antigenicity, plaque siz
e on IBRS-2 cells and single-step growth. Pigs were experimentally inf
ected with the parental virus, J1'73 strain, and viruses recovered fro
m cells transfected with the plasmids pSVLSJ1 and pSVLS00 [Inoue T, Ya
maguchi S, Saeki T, Sekiguchi K, J Gen Virol 71: 1835-1838 (1990)] cor
responding to the pathogenic (J1'73) and non-pathogenic (H/3'76) Japan
ese strains of the SVDV, respectively. All pigs inoculated with the vi
rus recovered from pSVLSJ1 produced clinical signs of similar severity
to those inoculated with the parental J1'73 strain. In contrast, pigs
inoculated with the virus recovered from pSVLS00 did not show any cli
nical signs. Viruses recovered from cells transfected with either pSVL
SJ1 or pSVLS00 therefore retained the in vitro characteristics and the
in vivo pathogenicity of their respective parental strains.