AN IMPROVED METHOD TO OBTAIN A SINGLE RECOMBINANT VASOACTIVE-INTESTINAL-PEPTIDE (VIP) ANALOG

The vasoactive intestinal peptide (VIP) is an ubiquitous peptide of gr eat potential for applications. Development of new bioactive VIP analo gs using production in recombinant E coli has been carried out in our laboratory. This work presents a new multimeric fusion protein express ing several VIP units separated by factor Xa cleavage site linkers. Th e steps leading from the affinity purification of the fusion protein a nd its processing by the factor Xa to the full characterization of the new bioactive improved VIP analog are also described. (C) Societe fra ncaise de biochimie et biologie moleculaire/Elsevier, Paris.