SELECTIVE NMR EXPERIMENTS ON MACROMOLECULES - IMPLEMENTATION AND ANALYSIS OF QUIET-NOESY

The QUIET-NOESY experiment (Zwahlen et al., J. Am. Chem Sec. 116, 362- 368, 1994) is applied to measure the mobility of the flexible extensio ns in the large aggregate (800 kDa) of a small heat-shock protein. The proper choices of the experimental protocol and parameters are discus sed in order to employ a simplified data analysis procedure. Further e xperimental verification of the proposed strategy is also presented us ing the cyclic peptide gramicidin S as a model compound. Under suitabl e conditions, the determinations based on the analysis of QUIET-NOESY data are affected to a negligible extent by the approximations that ar e introduced by the proposed approach. (C) 1998 Academic Press.