CHARACTERIZATION OF PROTEIN-HAPTEN CONJUGATES BY MASS-SPECTROMETRY

Authors
GOURLAOUEN N BOLBACH G FLORENTIN D GONNET F MARQUET A
Citation
N. Gourlaouen et al., CHARACTERIZATION OF PROTEIN-HAPTEN CONJUGATES BY MASS-SPECTROMETRY, COMPTES RENDUS DE L ACADEMIE DES SCIENCES SERIE II FASCICULE C-CHIMIE, 1(1), 1998, pp. 35-40
Citations number
13
Categorie Soggetti
Chemistry
Journal title
COMPTES RENDUS DE L ACADEMIE DES SCIENCES SERIE II FASCICULE C-CHIMIE → ACNP
ISSN journal
13871609
Volume
1
Issue
1
Year of publication
1998
Pages
35 - 40
Database
ISI
SICI code
1387-1609(1998)1:1<35:COPCBM>2.0.ZU;2-Y
Abstract
An hapten 1 designed for the production of catalytic antibodies was sy nthesized after coupling its precursor 2 to bovine serum albumin (BSA) . The conjugates BSA-2 and BSA-1 were characterized by MALDI mass spec trometry. This paper shows that besides the average number of molecule s bound to the protein deduced from the molecular ion peak shift, the MALDI technique can also give access to their distribution, by simulat ion of the peak.