IL-10 AND IL-4 COOPERATE TO NORMALIZE IN-VITRO IGA PRODUCTION IN IGA-DEFICIENT (IGAD) PATIENTS

In the present study we evaluated in vitro immunoglobulin production f rom IgAD individuals and healthy controls. Peripheral blood mononuclea r cells (PBMC) from IgAD and controls were cultured with anti-CD40 MoA b presented on a CDw32-transfected fibroblast cell line (CD40 system) in the presence of IL-10 IL-2, IL-4, transforming growth factor-beta ( TGF-beta) alone as well as of IL-10 in combination with each of the ot her three cytokines. Only ILIO added alone induced significant changes in baseline immunoglobulin production; marked increases in median sup ernatant levels of all three isotypes were observed in both groups. Th e most striking finding of this study was the synergizing effect of IL -4 on IEA production in the IgAD group when added with IL-10; median I gA supernatant level increased to a value superimposable on that found in the normal controls which remained about the same as when stimulat ed with IL-10 alone. The synergic effect of IL-4 and IL-10 was specifi c to the IgA isotype.