DEVELOPMENTAL-CHANGES IN THE CALCIUM SENSITIVITY OF RABBIT CRANIAL ARTERIES

The present experiments examine developmental changes in cerebrovascul ar Ca2+ sensitivity. Common carotid (COM), basilar (BAS) and femoral ( FA) arteries from adult (n = 16), 8- to 9-day-old (n = 15) and 24- to 25-day-old rabbits (n = 12) were denuded of the endothelium and permea bilized with beta-escin. Bath calcium concentrations were controlled v ia EGTA-Ca2+ buffer solutions. Adult pCa-force relations were right-sh ifted relative to those of 8-to 9-day-old rabbits but were similar to those of 34- to 25-day-olds. Adult pD(2) (-log ED50) values for Ca2+ a veraged 6.36 +/- 0.03 (COM), 6.77 +/- 0.04 (BAS) and 6.40 +/- 0.04 (FB ). Corresponding 8-to 9-day-old values were 6.85 +/- 0.03, 7.08 +/- 0. 08 and 6.76 +/- 0.05. In all arteries studied, the addition of 5-hydro xytryptamine (5-HT) subsequent to contraction by a constant submaximal (EC30) concentration of Ca2+ produced a dose-dependent and GDP beta S- sensitive increase in tension attributable to an increase in Ca2+ sens itivity. The magnitudes of 5-HT-induced increases in Ca2+ sensitivity were significantly greatest in 8-to 9-day-old rabbits, intermediate in 24-to 25-day-old rabbits, and least in adults. GTP gamma S mimicked t he effects of 5-HT and prevented further increases in Ca2+ sensitivity induced by 5-HT in all arteries from all age groups. GDP beta S compl etely reversed all effects of 5-HT on Ca2+ sensitivity. From these dat a we conclude that baseline Ca2+ sensitivity is elevated in newborn re lative to adult rabbits, at least in femoral, common carotid and basil ar arteries. In these arteries, 5-HT can increase Ca2+ sensitivity via a G-protein-dependent mechanism which is more effective in neonatal t han adult arteries. These effects of maturation on vascular Ca2+ sensi tivity may play an important role in developmental changes in vascular reactivity.