GAIN OF CHROMOSOME-7 MARKS THE PROGRESSION FROM INDOLENT TO AGGRESSIVE FOLLICLE CENTER LYMPHOMA AND IS A COMMON FINDING IN PATIENTS WITH DIFFUSE LARGE B-CELL LYMPHOMA - A STUDY BY FISH

Citation
P. Bernell et al., GAIN OF CHROMOSOME-7 MARKS THE PROGRESSION FROM INDOLENT TO AGGRESSIVE FOLLICLE CENTER LYMPHOMA AND IS A COMMON FINDING IN PATIENTS WITH DIFFUSE LARGE B-CELL LYMPHOMA - A STUDY BY FISH, British Journal of Haematology, 101(3), 1998, pp. 487-491
Citations number
13
Categorie Soggetti
Hematology
ISSN journal
00071048
Volume
101
Issue
3
Year of publication
1998
Pages
487 - 491
Database
ISI
SICI code
0007-1048(1998)101:3<487:GOCMTP>2.0.ZU;2-7
Abstract
Gain of chromosome 7 represents one of the most frequent cytogenetic f indings in B-cell lymphomas with a follicular growth pattern, We used fluorescence in situ hybridization (FISH) and a probe specifying chrom osome 7 on lymph node imprints and/or bone marrow (BM and peripheral b lood (PB) smears from six consecutive patients with follicle centre ly mphomas (FCLs) grade I or II (low-grade lymphomas), four patients with FCLs grade III and 11 patients with diffuse large B-cell lymphomas (D LBCLs) thigh-grade lymphomas). We found gains of chromosome 7 in 14/18 successfully analysed cases (i.e. 2/6 FCLs grade I-II, 3/3 FLCs grade III and in 9/9 DLBCLs) using lymph node imprints. Moreover, the FISH technique demonstrated gains of chromosome 7 in 1/4 BM and 0/4 PB samp les from FCLs grade I-II, in 2/4 BM and 2/4 PB specimens from FCLs gra de III and in 4/9 BM and 219 PB samples from the DLBCLs. In contrast, morphologically recognizable lymphoma cells were seen in only 1/4 BM a nd 0/4 PB samples from the FCLs grade III and in 1/11 BM and 1/11 PB s amples from the DLBCLs. We conclude that: (i) gain of chromosome 7 mar ks the progression from indolent to aggressive FCL and would appear to be a common finding in patients with FCLs grade III and in DLBCLs, (i i) clonal lymphoid cells occur frequently in BM and PB in high-grade l ymphomas, making traditional staging by cytomorphology uncertain, and (iii) using gains of chromosome 7 as a marker of lymphoma cells, FISH is a useful method to detect minimal residual disease in FCLs grade II I and DLBCLs.