M. Arahira et al., MOLECULAR-CLONING AND EXPRESSION PATTERNS OF CU ZN-SUPEROXIDE DISMUTASES IN DEVELOPING SOYBEAN SEEDS/, Bioscience, biotechnology, and biochemistry, 62(5), 1998, pp. 1018-1021
Assuming that the amount of superoxide radicals generated in vivo corr
elates with the production of ergastic substances such as storage prot
eins, the coordinated response of detoxication enzymes such as superox
ide dismutases is largely exploited to understand the self-defense sys
tems of plant. Here we examined expression of the genes for superoxide
dismutases during seed development of soybean. The cDNAs encoding a c
ytosolic copper/zinc form and an iron form of the above enzyme have be
en cloned and then employed as probes, separately. Northern blotting r
esults suggested that both superoxide dismutase mRNAs are expressed at
the maximum level, preceding a developmental stage when mRNA encoding
glycinin, soybean 11S-storage protein, at the maximum.