CDNA CLONING, EXPRESSION, AND CHARACTERIZATION OF THE HUMAN BIFUNCTIONAL ATP SULFURYLASE ADENOSINE 5'-PHOSPHOSULFATE KINASE ENZYME/

Authors
YANAGISAWA K SAKAKIBARA Y SUIKO M TAKAMI Y NAKAYAMA T NAKAJIMA H TAKAYANAGI K NATORI Y LIU MC
Citation
K. Yanagisawa et al., CDNA CLONING, EXPRESSION, AND CHARACTERIZATION OF THE HUMAN BIFUNCTIONAL ATP SULFURYLASE ADENOSINE 5'-PHOSPHOSULFATE KINASE ENZYME/, Bioscience, biotechnology, and biochemistry, 62(5), 1998, pp. 1037-1040
Citations number
18
Categorie Soggetti
Biology,Agriculture,"Biothechnology & Applied Migrobiology","Food Science & Tenology
Journal title
Bioscience, biotechnology, and biochemistry → ACNP
ISSN journal
09168451
Volume
62
Issue
5
Year of publication
1998
Pages
1037 - 1040
Database
ISI
SICI code
0916-8451(1998)62:5<1037:CCEACO>2.0.ZU;2-7
Abstract
A cDNA encoding the human bifunctional ATP sulfurylase/adenosine 5'-ph osphosulfate (APS) kinase was cloned and sequenced. The enzyme contain s an APS kinase domain in its N-terminal portion and an ATP sulfurylas e domain in its C-terminal portion. Recombinant full-length enzyme and its constituent APS kinase and ATP sulfurylase domains were individua lly expressed, purified, and shown to have their respective enzymatic activities.