STUDIES ON THE LOSS OF INFECTIVITY OF LIVE TYPE-3 POLIOVACCINE ON STORAGE

The Children's Vaccine Initiative established a Product Development Gr oup in 1991 to help in the development of oral poliovaccines of improv ed thermostability. Under the auspices oi this group, the effect of di fferent stabilizers on the loss oi infectivity of commercial monovalen t type 3 live poliovaccine bulks at 37 degrees C, 42 degrees C and 45 degrees C has been examined. The only effective conditions were the ma intenance of the pH at 6.5, and the use of deuterium oxide at a concen tration of 90%. Ail other additives, including detergents, albumin, an tiviral compounds, sucrose and magnesium chloride, alone or in combina tion either had no effect or increased the rate oi loss of infectivity . (C) 1998 The International Association of Biological Standardization .