Dp. Knight et al., IN-VITRO FORMATION BY REVERSE DIALYSIS OF COLLAGEN GELS CONTAINING HIGHLY ORIENTED ARRAYS OF FIBRILS, Journal of biomedical materials research, 41(2), 1998, pp. 185-191
Acid extracts of rat tail tendon were subjected to reverse dialysis ag
ainst 0.5% PEG at 4 degrees C in an attempt to induce liquid crystalli
zation. After 48 h, gel and fibril formation were initiated by continu
ing dialysis at 20 degrees C against the same PEG solution adjusted to
pH 7.4. The inclusion of calcium- or magnesium chloride (final concen
tration 0.3-33 mM) in the collagen solution before dialysis resulted i
n strongly birefringent gels that showed a progressive rotation of the
slow axis of birefringence with increasing distance from the lateral
margin of the gel. The gels contained fibers running predominantly in
the plane of the flattened gel and crossing at angles of between 55 de
grees and 90 degrees. We suggest that liquid crystallization is respon
sible for this phenomenon and that it might be possible to exploit thi
s to produce materials for tissue engineering. (C) 1998 John Wiley & S
ons, Inc.