IMMUNE-RESPONSES AND PROTECTIVE EFFICACY OF RECOMBINANT BACULOVIRUS-EXPRESSED GLYCOPROTEINS OF EQUINE HERPESVIRUS-1 (EHV-1) GB, GC AND GD ALONE OR IN COMBINATIONS IN BALB C MICE/
P. Packiarajah et al., IMMUNE-RESPONSES AND PROTECTIVE EFFICACY OF RECOMBINANT BACULOVIRUS-EXPRESSED GLYCOPROTEINS OF EQUINE HERPESVIRUS-1 (EHV-1) GB, GC AND GD ALONE OR IN COMBINATIONS IN BALB C MICE/, Veterinary microbiology, 61(4), 1998, pp. 261-278
Baculovirus-expressed glycoproteins of EHV-1 gB, gC and go alone or in
combination evoked antibody responses and protected vaccinated mice a
gainst a challenge with EHV-1 gB, go, gB + gC, gB + gD and gC + gD eli
cited very high levels of ELISA antibodies while gC and gC + gD elicit
ed high levels of virus neutralising antibodies. Western blotting demo
nstrated that the antibodies produced were not only specific for the b
aculovirus-expressed glycoproteins gB, gC and go, but also highly spec
ific for each EHV-1glycoprotein. Vaccination of mice with gB or go pre
vented clinical signs of infection in mice challenged with EHV-1 and a
ll vaccinated groups of mice except controls shelved a rapid clearance
of virus from the lungs and a reduction in lesions characteristic of
herpesviruses in the lungs post-challenge. Notably, the lungs of mice
vaccinated with gB, go or gB + gD and challenged with EHV-1showed prom
inent peribronchiolar and perivascular aggregations of mononuclear cel
ls, predominantly lymphocytes. Immunocytochemical staining of these se
ctions showed large numbers of T cells, suggesting an active role for
these cells at the site of virus replication post-challenge. (C) 1998
Elsevier Science B.V.