C. Li et al., A SIMPLIFIED AND PRACTICAL METHOD FOR METHYLATION ANALYSIS OF POLYSACCHARIDES, Indian journal of chemistry. Sect. B: organic chemistry, including medical chemistry, 37(4), 1998, pp. 323-324
Butyllithium is directly added to the solution of polysaccharides in d
imethyl sulfoxide. The methylsulfinyl carbanion, as sugar-alkoxide-for
ming reagent, is generated in the solution. All the operations are per
formed as one-pot reaction. Five polysaccharides from different tradit
ional Chinese medicines and four dextrans have been methylatated and s
atisfactory results achieved.