SPATIAL PHOTOSELECTION OF SINGLE MOLECULES ON THE SURFACE OF SPHERICAL MICROCAVITIES

Citation
N. Lermer et al., SPATIAL PHOTOSELECTION OF SINGLE MOLECULES ON THE SURFACE OF SPHERICAL MICROCAVITIES, Optics letters, 23(12), 1998, pp. 951-953
Citations number
18
Categorie Soggetti
Optics
Journal title
ISSN journal
01469592
Volume
23
Issue
12
Year of publication
1998
Pages
951 - 953
Database
ISI
SICI code
0146-9592(1998)23:12<951:SPOSMO>2.0.ZU;2-L
Abstract
We show that ultrasensitive microdroplet-stream fluorescence technique s combined with surfactant forms of Rhodamine dyes can be used to prob e single molecules on the surfaces of spherical microcavities. Individ ual octadecyl Rhodamine B molecules, shown previously by ensemble meas urements to be localized and oriented at the surfaces of liquid micros pheres, were spatially photoselected primarily along great circles lyi ng perpendicular or parallel to the detection axis by use of polarized laser excitation. A polarization dependence is observed in the distri bution of single-molecule fluorescence amplitudes that can be interpre ted qualitatively in terms of position-dependent fluorescence-collecti on efficiencies. (C) 1998 Optical Society of America.