K. Sakano et al., DEGRADATION OF ENDOGENOUS ORGANIC-ACIDS INDUCED BY P-I UPTAKE IN CATHARANTHUS-ROSEUS CELLS - INVOLVEMENT OF THE BIOCHEMICAL PH-STAT, Plant and Cell Physiology, 39(6), 1998, pp. 615-619
We have previously shown that inorganic orthophosphate (P-i) uptake by
Catharanthus roseus cells proceeds by a proton/P-i cotransport mechan
ism [Sakano (1990) Plant Physiol. 93: 479] that acidifies the cytoplas
m [Sakano et al. (1992) Plant Physiol. 99: 672]. In the present study,
we analyzed changes in the content of endogenous organic acids, carbo
n dioxide evolution, and oxygen consumption upon P-i application. The
results are consistent with the operation of the biochemical ps-stat m
echanism [Davies (1986) Physiol. Plant. 67: 702] during and after P-i
uptake.