FLOW CYTOMETRIC DNA ANALYSES OF BENIGN BREAST-LESIONS DETECTED BY SCREENING MAMMOGRAPHY

There is little information regarding flow cytometric DNA analyses of benign breast lesions. This prospective study consists of mammographic and pathological correlation of DNA flow cytometric analyses of speci men mammography-guided fine-needle aspirates (FNAs) of 189 consecutive benign breast lesions and 114 FNAs of adjacent normal tissue as a con trol. Clinical follow-up was also performed. Aneuploidy was detected i n 14 of 189 (7%) benign lesion specimen mammography-guided FNAs and in only 1 of 114 (0.9%) FNAs of adjacent normal tissue (P = 0.01), Aneup loidy was detected in two (33%) benign intramammary lymph nodes compar ed with four (12%) benign lesions with atypia, one benign lesion (3%) with hyperplasia, four benign lesions (10%) with adenosis, and three ( 4%) other benign lesions (P = 0.01), There were no significant associa tions between DNA content and S-phase percentage and patient age, mamm ographic appearance, or extent. During a median follow-up of 40 months (range, 6-84 months), 2 of 13 (15%) patients with aneuploid benign le sions developed ipsilateral breast carcinoma compared with 5 of 175 (3 %) patients with diploid benign lesions (odds ratio, 6.18; 95% confide nce interval, 1.08-35.56). Our data suggest that aneuploidy, which is detected in a variety of benign breast lesions, may be associated with a higher risk of development of breast carcinoma. The combined techni ques of specimen mammography-guided fine-needle aspiration and flow cy tometry provide a practical translational research method for the stud y of benign breast disease.