PLASMA-CONCENTRATIONS OF RISPERIDONE AND ITS 9-HYDROXY METABOLITE ANDTHEIR RELATIONSHIP TO DOSE IN SCHIZOPHRENIC-PATIENTS - SIMULTANEOUS DETERMINATION BY A HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH ELECTROCHEMICAL DETECTION
M. Aravagiri et al., PLASMA-CONCENTRATIONS OF RISPERIDONE AND ITS 9-HYDROXY METABOLITE ANDTHEIR RELATIONSHIP TO DOSE IN SCHIZOPHRENIC-PATIENTS - SIMULTANEOUS DETERMINATION BY A HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH ELECTROCHEMICAL DETECTION, Pharmacopsychiatry, 31(3), 1998, pp. 102-109
A simple, sensitive and accurate method for the simultaneous determina
tion of risperidone (RSP) and its 9-hydroxy metabolite (9-OH-RSP) in h
uman plasma is described. The relationship between dose of RSP and the
plasma concentration of RSP and 9-OH-RSP in a clinical situation is d
iscussed. Both compounds were isolated from plasma by a simple one-ste
p liquid-liquid extraction with 15% methylene chloride in pentane. Hig
h-performance liquid chromatography separations were made on a cyano c
olumn and the compounds were detected by electrochemical detector. The
method had sufficient sensitivity to determine RSP and 9-OH-RSP accur
ately at concentrations as low as 0.25 ng/ml when 1 ml of plasma is us
ed for the analysis. The assay determinations were accurate, precise a
nd consistent with a coefficient of variation less than 15%. Commonly
co-administered drugs and other antipsychotics did not interfere with
the analysis of either RSP or 9-OH-RSP There were large variations in
inter- and intra-individual values of plasma concentrations of RSP and
9-OH-RSP. The 9-OH-RSP appears to be the major circulating active moi
ety and its plasma concentrations were, on the average 22 fold higher
than that of RSP in schizophrenic patients treated with RSP. The ratio
of RSP/9-OH-RSP concentrations suggested that three of the patients m
ay have deficiency in cytochrome P450 enzyme CYP 2D6. The plasma conce
ntrations of RSP showed a weak relationship with the administered dail
y oral dose (r = 0.4684, p = 0.01, n = 215). However, there was a good
relationship between the daily dose of RSP and the plasma concentrati
on of 9-OH-RSP (r = 0.6654, p = 0.01, n = 280) or the total active moi
ety, sum of RSP and 9-OH-RSP concentrations (r = 0.7041, p = 0.0005, n
= 280). The measurement of the total active moiety in plasma of schiz
ophrenic patients may be useful for assessing the relationship between
dose and plasma concentration and dose and clinical outcome of patien
ts rather than measuring RSP alone.