A CALCIUM-PUMP AT THE HIGHER-PLANT NUCLEAR-ENVELOPE

Evidence for a Ca2+-pump at the nuclear envelope (NE) in plant cells h as been obtained using confocal and electron microscope immunocytochem istry and antibodies raised to a plant homologue of the mammalian SERC A pump, This is the first evidence suggesting an NE Ca2+-pump in plant s, In addition to being localised,with the NE in interphase, the antig en was localised to membrane derived from the NE and associated ER dur ing mitosis, correlating with known Ca2+-pools. The work suggests that a SERCA pump is present at the NE of plant as well as animal cells. ( C) 1998 Federation of European Biochemical Societies.