Xn. Xuan et al., BIOLOGICAL AND IMMUNOGENIC PROPERTIES OF RABIES VIRUS GLYCOPROTEIN EXPRESSED BY CANINE HERPESVIRUS VECTOR, Vaccine, 16(9-10), 1998, pp. 969-976
Citations number
48
Categorie Soggetti
Veterinary Sciences",Immunology,"Medicine, Research & Experimental
In order to evaluate whether canine herpesvirus (CHV) could be used as
a live vector for the expression of heterologous immunogenes, we cons
tructed a recombinant canine herpesvirus (CHV) expressing glycoprotein
(G protein) of rabies virus (RV). The gene of G protein was inserted
within the thymidine kinase gene of CHV YP11mu strain under the contro
l of the human cytomegalovirus immediate early promoter. The G protein
expressed by the recombinant CHV was processed and transported to the
cell surface as in RV infected cells, and showed the same biological
activities such as low pH dependent cell fusion and hemadsorption. The
antigenic authenticity of the recombinant G protein was confirmed by
a panel of monoclonal antibodies specific for G protein. Dogs inoculat
ed intranasally with the recombinant CHV produced higher titres of vir
us neutralizing antibodies against RV than those inoculated with a com
mercial, inactivated rabies vaccine, These results suggest that the CH
V recombinant expressing G protein can be used as a vaccine to control
canine rabies and that CHV may be useful as a vector to develop live
recombinant vaccines against other infectious diseases in dogs. (C) 19
98 Elsevier Science Ltd. All rights reserved.