Vm. Ratnamohan et al., REMOVAL OF INHIBITORS OF CSF-PCR TO IMPROVE DIAGNOSIS OF HERPESVIRAL ENCEPHALITIS, Journal of virological methods, 72(1), 1998, pp. 59-65
Citations number
17
Categorie Soggetti
Virology,"Biochemical Research Methods","Biothechnology & Applied Migrobiology
CSF-PCR is currently the most sensitive test to diagnose encephalitis
due to cytomegalovirus or herpes simplex virus. However, false negativ
e results sometimes arise due to inhibitors in CSF. We have shown that
the inhibitory effects may be due to increased levels of proteins and
increased cell numbers, but are not due to cellular DNA. Simple techn
iques were used to remove the inhibitors and successfully apply these
methods to CSF specimens that gave equivocal results when tested untre
ated. (C) 1998 Elsevier Science B.V. All rights reserved.