NOVEL METHOD OF DETECTING MISMATCH ON DNA BY USING PHOTOINDUCED ELECTRON-TRANSFER THROUGH DNA

Novel methodology for detecting a defect on double stranded DNA was pr esented, the principle being based on the dependence of the efficiency of photo-induced electron transfer through double stranded DNA on the structural disorders in the base stacking. The efficiency of photo-in duced electron transfer between tetrakis(N-methylpyridinium-4-yl)-21H, 23H-porphine tetrachloride and N,N'-dimethyl-4,7-phenanthrolinium dich loride on double stranded DNA was changed by several base-mispairing o n the double stranded DNA. This phenomenon will be useful for the deve lopment of the convenient method of detecting mismatch on DNA duplex i n homogeneous solution. (C) 1998 Elsevier Science B.V.