AFFINITY ADSORPTION SEPARATION OF MUTAGENIC MOLECULES BY POLYACRYLAMIDE HYDROGELS COMPRISING DOUBLE-STRANDED DNA

Polyacrylamide hydrogels comprising double-stranded DNA were synthesiz ed and applied as the absorbent for DNA-binding mutagenic molecules. V inyl groups were immobilized on salmon sperm DNA via photoreaction bet ween DNA and a vinyl derivative of psoralen. The resulting DNA macromo nomer was fed into the polymerization system of acrylamide and N,N'-me thylenebisacrylamide to give a hybrid hydrogel between DNA and polyacr ylamide. Adsorption functions of the DNA hydrogel were studied for a v ariety of DNA-binding drugs and dyes. (C) 1998 Elsevier Science B.V.